A cikin 1509, babban yakin Diu (1509) ya faru tsakanin Portuguese da rundunar hadin gwiwa na Sultan Gujarat,Mamluk Sultanate naMasar , Zamorin na Calicut tare da goyon bayan Daular Ottoman .Tun daga 1517, Ottomans sun yi ƙoƙari su haɗa sojoji da Gujarat don yakar Portuguese daga Bahar Maliya da kuma yankinIndiya .Selman Reis ne ya shigar da sojojin da ke goyon bayan Ottoman karkashin Kyaftin Hoca Sefer a Diu.Diu a Gujarat (yanzu jiha ce a yammacin Indiya), yana tare da Surat, ɗaya daga cikin manyan wuraren samar da kayan yaji zuwa Masar Ottoman a lokacin.Duk da haka, shiga tsakani na Portuguese ya dakile wannan ciniki ta hanyar sarrafa zirga-zirgar ababen hawa a cikin Bahar Maliya.A cikin 1530, ' yan Venetian ba su iya samun kayan yaji ta Masar ba.Yakin Diu ya faru ne a lokacin da sojojin masarautar Gujarat karkashin Khadjar Safar tare da taimakon dakarun daular Usmaniyya suka yi yunkurin kwace birnin Diu a shekara ta 1538, sannan Turawan Portugal suka rike.Portuguese din sun yi nasarar tsayayya da kewaye na watanni hudu.Rashin nasarar da sojojin Turkiyya da na Gujarati suka yi a Diu ya nuna babban koma baya a shirin Ottoman na fadada tasirinsu zuwa Tekun Indiya.Ba tare da tushe mai dacewa ko abokan tarayya ba, rashin nasara a Diu yana nufin Ottomans sun kasa ci gaba da yakin neman zabe a Indiya, suna barin Portuguese ba tare da hamayya ba a yammacin gabar Indiya.Turkawa Ottoman ba za su sake aika babban armada zuwa Indiya ba.