Babban Siege na Malta ya faru ne a cikin 1565 lokacin da Daular Ottoman ta yi ƙoƙari ta mamaye tsibirin Malta, sannan mai kula da Asibitin Knights ya riƙe.Sifen ya dau kusan watanni hudu, daga 18 ga Mayu zuwa 11 ga Satumba, 1565.Asibitin Knights yana da hedikwata a Malta tun 1530, bayan da aka kore shi daga Rhodes, da kuma Ottomans, a 1522, bayan da aka kewaye Rhodes.Daular Usmaniyya ta fara yunkurin daukar Malta a shekara ta 1551 amma ta kasa.A cikin 1565, Suleiman Mai Girma, Sultan Ottoman, ya yi ƙoƙari na biyu don ɗaukar Malta.Knights, wadanda adadinsu ya kai kusan 500 tare da sojoji kusan 6,000, sun yi tsayin daka da kewaye kuma suka fatattaki maharan.Wannan nasara ta zama ɗaya daga cikin abubuwan da aka fi sha'awa a Turai na ƙarni na goma sha shida, har Voltaire ya ce: "Babu wani abu da aka fi sani fiye da kewayen Malta."Babu shakka hakan ya taimaka wajen rugujewar fahimtar da Turawa suka yi na rashin nasara a Ottoman, duk da cewa an ci gaba da gwabza fada tsakanin tekun Mediterrenean tsakanin kawancen Kiristoci da Turkawa Musulmi tsawon shekaru.Wannan kawanya dai ita ce kololuwar wata fafatawar da ta kunno kai tsakanin kawancen kiristoci da daular Musulunci ta Daular Usmaniyya na mallakar tekun Bahar Rum, gasar da ta hada da harin da Turkiyya ta kai a Malta a shekara ta 1551, da kuma lalata daular Usmaniyya ta rundunar sojojin kawance ta Kiristoci a yakin Djerba. 1560, da kuma babban nasara Ottoman a yakin Lepanto a 1571.