Bayan yakin 1547-49 da Toungoo, Sarkin Ayutthaya Maha Chakkrahat ya gina kariyar babban birninsa a shirye-shiryen yaki da Burma.Yaƙin 1547-49 ya ƙare a cikin nasarar tsaro ta Siamese da kiyaye 'yancin kai na Siamese.Duk da haka, burin Baynnaung na yanki ya sa Chakkraphat ya shirya wani hari.Waɗannan shirye-shiryen sun haɗa da ƙidayar jama'a waɗanda suka shirya duk masu iya zuwa yaƙi.Gwamnati dai ta kwashe makamai da dabbobi a shirye-shiryen wani gagarumin yakin yaki, sannan Chakraphat ya kama wasu fararen giwaye guda bakwai domin samun sa'a.Labarin shirye-shiryen sarki Ayutthaya ya bazu cikin sauri, daga ƙarshe ya isa Burma.Bayinnaung ya yi nasarar karbe birnin Chiang Mai a cikin daular Lan Na da ke kusa a cikin 1556. Yunkurin da ya biyo baya ya bar yawancin arewacin Siam karkashin ikon Burma.Wannan ya bar masarautar Chakkraphat a cikin wani yanayi mara kyau, yana fuskantar yankin abokan gaba a arewa da yamma.Bayinnaung daga baya ya bukaci biyu daga cikin fararen giwayen Sarki Chakkrahat a matsayin girmamawa ga daular Toungoo mai tasowa.Chakkrahat ya ƙi, wanda ya kai ga mamayewar Burma na biyu na Masarautar Ayutthaya.Sojojin Bayinnaung sun yi tattaki zuwa Ayutthaya.A can, sansanin Siamese ya ajiye su na tsawon makonni, tare da taimakon jiragen ruwa na Portugal guda uku da batura na bindigogi a tashar jiragen ruwa.A ƙarshe maharan sun kama jiragen ruwa da batura na Portuguese a ranar 7 ga Fabrairun 1564, bayan haka katangar ta fadi nan da nan.^[43] Tare da dakaru mai karfi 60,000 a yanzu hade da sojojin Phitsanulok, Bayinnaung ya isa ganuwar birnin Ayutthaya, inda suka yi ruwan bama-bamai a birnin.Ko da yake sun fi karfinsu, Burma ba su sami damar kama Ayutthaya ba, amma sun bukaci Sarkin Siamese ya fito daga birnin a karkashin tutar sasantawa don yin shawarwarin zaman lafiya.Ganin cewa 'yan kasarsa ba za su iya daukar dogon lokaci ba, Chakkraphat ya yi shawarwarin zaman lafiya, amma a farashi mai yawa.A musanya ja da baya na sojojin Burma, Bayinnaung ya ɗauki Yarima Ramesuan (ɗan Chakraphat), Phraya Chakri, da Phraya Sunthorn Songkhram tare da shi zuwa Burma a matsayin garkuwa, da kuma fararen giwayen Siamese huɗu.Mahathamraja, ko da yake ya ci amana, an bar shi a matsayin mai mulkin Phitsanulok da mataimakin Siam.Masarautar Ayutthaya ta zama mai mulkin daular Toungoo, wacce ake buƙata ta ba da giwaye talatin da garken azurfa ɗari uku ga Burma kowace shekara.